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The effects of plant extracts on microbial community structure in a rumen-simulating continous-culture system as revealed by molecular profilingKušar, Darja, 1977- ...An in vitro study in dual-flow continous-culture fermentors was conducted with two different concentrations of monesin, cinnamaldehyde or garlic extract added to 1:1 forage-to-concentrate diet in ... order to determine their effects on selected rumen bacterial populations. Samples were subjected to total DNA extraction, restriction analysis of PCR amplified parts of 16S rRNA genes (ARDRA) and subsequent analysis of the restriction profiles by lab-on-chip technology with the Agilent's Bioanalyser 2100. EUB 338-BacPre primer pair was used to select for the bacteria from the genera Bacteroides, Porphyromonas and Prevotella, especially the latter representing the dominant Gram-negative bacterial population in the rumen. Preliminary results of HaeIII restriction analysis show that effects of monesin, cinnamaldehyde and garlic extract on the BacPre targeted ruminal bacteria are somewhat different in regard to targeted populations and to the nature of the effect. Garlic extract was found to trigger the most intensive changes in the structure of the BacPre targeted population. Comparison of the in silico restriction analysis of BacPre sequences deposited in different DNA databanks and of the results of performed amplified ribosomal DNA restriction analysis showed differences between the predicted and obtained HaeIII restriction profiles, and suggested the presence of novel, still unknown Prevotella populations in studied samples.Source: Folia microbiologica. - ISSN 0015-5632 (Letn. 49, št. 2, 2004, str. 151-155)Type of material - article, component partPublish date - 2004Language - englishCOBISS.SI-ID - 1651080
Author
Kušar, Darja, 1977- |
Banjac, Marko |
Calsamiglia, Sergio |
Busquet, M. |
Kamel, Chris |
Avguštin, Gorazd
Topics
mikrobiologija |
bakterije |
vamp |
rastlinski izvlečki |
molekularna genetika
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Kušar, Darja, 1977- | 24296 |
Banjac, Marko | 28000 |
Calsamiglia, Sergio | ![]() |
Busquet, M. | ![]() |
Kamel, Chris | ![]() |
Avguštin, Gorazd | 08320 |
Source: Personal bibliographies
and: SICRIS
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