Vpliv cinkovih ionov na rast kvasovk Saccharomyces cerevisiae = Effect of zinc ions on growth of yeast Saccharomyces cerevisiae
Paš, Maja ; Batič, Martin ; Raspor, Peter
Z mikroelementi obogatena kvasna biomasa predstavlja nov naravni vir mikroelementov v řloveǴki prehrani. Kvasovke lahko pod dolořenimi pogoji veÍejo mikroelemente z oblikovanjem organskih vezi v ...mnogo viǴjih koncentracijah kot so obiřajne. Dolořena mnoÍina Zn2+ ionov v rastnem mediju je esencialna za normalno rast in razmnoÍevanje kvasovk, visoke koncentracije Zn2+ ionov pa delujejo nanje toksiřno. Ugotavljali smo vpliv poveřanih koliřinZn2+ ionov, ki smo jih dodali v polsintetiřno tekoře kvasno gojiǴře, narast kvasovke Saccharomyces cerevisiae med ǴarÍnimi aerobnimi kultivacijami v laboratorijskem bioreaktorju. Edini limitni faktor pri izvedbi vseh kultivacij je bila mnoÍina Zn2+ ionov v gojiǴřu, hkrati pa so bili pogoji bioprocesa za vse kultivacije enaki. Opravili smo "ON LINE" meritve: pH, pO2, redoks potencial, % O2 v izstopnem zraku, optiřna gostota. Med bioprocesi smo vsake Ǵtiri ure jemali vzorce bioprocesnih brozg in v njih dolořili koncentraciji neporabljene glukoze ter cinka ("OFF LINE" analize). Optimalna koncentracija Zn2+ ionov za rast in razmnoÍevanje kvasovk Saccharomyces cerevisiae je bila 240 mg/dm3, viǴje koncentracije Zn2+ ionov pa zavirajo rastkvasovk.
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