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Aerobni bioproces rasti kvasovke Saccharomyces cerevisiae in akumulacija RNK : diplomska naloga = Aerobic bioprocess of growth of yeast Saccharomyces cerevisiae and accumulation of RNA : graduation thesisLikar, PrimožPostavitev aerobnega bioprocesa rasti kvasovke Saccharomyces cerevisiae - ZIM 753 za povišano akumulacijo RNK v celicah, je vključevala izbor in optimizacijo gojišča in bioprocesnih parametrov. Na ... osnovi presejalne metode, ki je vključevala štiri različna razmerja C:N (5:1; 10:1; 25:1; 50:1) in začetne pH (2, 4, 6, 8) gojišča so bili za bioproces v večjem merilu izbrani naslednji parametri: razmerje C:N 5:1, začetni pH 4, trajanje bioprocesa 15 ur, temperatura 29 °C, pretok zraka 2 vvm in mešanje 250 obr./min. Med bioprocesom v 10 l pilotnem bioreaktorju so potekale "on line" in "off line" meritve pH, suhe biomase, RNK, proteinov, O2, CO2, glukoze, dušika in organskih kislin. V bioprocesu rasti kvasovke Saccharomyces cerevisiae - ZIM 753 smo iz 38 g/l glukoze dobili 6,25 g/l biomase in 450 mg/l RNK v brozgi, ob maksimalni specifični hitrosti rasti 0,24 h-1. Delež proteinov v suhi biomasi je znašal 39%. Kvasovka ima v postavljenem bioprocesu afiniteto do glukoze (Ks) 3,00 g/l pri tem pa porablja 0,17 g glukoze za vzdrževanje osnovnega metabolizma kvasnih celic na uro (ms). Specifična hitrost respiracije (qo) je bila 0,12 h-1 v ta namen pa je potrebno za doseganje Y'x/s0,15 zagotoviti maksimalno stopnjo prenosa kisika (OTR) 975 mg O2/lh.Type of material - undergraduate thesis ; adult, seriousPublication and manufacture - Ljubljana : [BF, Oddelek za živilstvo] : [P. Likar], 1999Language - slovenianCOBISS.SI-ID - 2179960
Author
Likar, Primož
Other authors
Batič, Martin |
Klofutar, Cveto
Topics
kvasovke |
Saccharomyces cerevisiae |
šaržni bioproces |
biomasa |
akumulacija RNK |
sestava gojišča |
vpliv pH |
bioprocesni parametri |
RNK ojačevalci arome |
RNK ojačevalci arome |
yeasts |
Saccharomyces cerevisiae |
bath bioprocess |
biomass |
accumulation of RNA |
medium composition |
pH influence |
bioprocess parameters |
RNA flavor enhancers |
RNA flavor enhancers
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Library | Call number – location, accession no. ... | Copy status |
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BF, Dept. of Food Science and Technology, Lj. | Hodnik DN 899 IN: 0011333 |
available - outside loan, loan period: 1 months |
BF, Dept. of Food Science and Technology, Lj. | Hodnik DN 899 b IN: 0013638 |
available - outside loan, loan period: 1 months |
BF, Dept. of Food Science and Technology, Lj. | Hodnik DN 899 a IN: 0011334 |
available - outside loan, loan period: 1 months |
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Database name | Field | Year |
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Batič, Martin | 07469 |
Klofutar, Cveto | 00177 |
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