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Mehanizem transporta glukoze v osmotskem šoku v inzulindko rezistentnih 3T3-L1 adipocitih = Mechanism of osmotic shock-induced glucose transport in insulin resistant 3T3-L1 adipocytes : doctoral dissertationJanež, Andrej, 1971-Osmotic shock treatment of 3T3-L1 adipocytes causes an increase in glucose transport activity and translocation of GLUT-4 protein similar to that elicited by insulin treatment. Insulin stimulation of ... GLUT-4 translocation andglucose transport activity was completely inhibited by wortmannin, however,activation by osmotic shock was only partialy blocked. Additionally, we have found that the newly identified insulin rcceptor substrate (IRS) Gab-1(Grb2-associated binder-1) is tyrosine phosphorylated following sorbitol stimulation. Treatment of cells with the tyrosine kinase inhibitor genestin inhibited osmotic shockstimulated Gab-1 phosphorylation, as well as shock-induced glucose transport. rurthennore, pretreatment with the selective Src family kinase inhibitor PP2 completely inhibited the ability of sorbitol treatment to cause tyrosine phosphorylation of Gab-1. We have also shown that microinjection of anti-Gab-1 antibody inhibits osmotic shock-induced GLUT-4 translocation. Furthermore, phosphorylated Gab-1 binds and activates phosphatidylinositol 3-kinase (PI 3-kinase) in response to osmotic shock. The PI 3-kinase activity associated with Gab-1 was 82% of that associated with antiphosphotyrosine, indicating that Gab-I is the major site for PI 3-kinase recruitment following osmotic shock stimulation. (Abstract truncated at 2000 characters)Type of material - dissertationPublication and manufacture - Ljubljana : [A. Janež], 2000Language - english, slovenianCOBISS.SI-ID - 2227220
Author
Janež, Andrej, 1971-
Other authors
Kocijančič, Andreja, 1942-2021 |
Olefsky, Jerrold M.
Topics
Monosaccharide transport proteins |
Physiology |
Monosaccharide transport proteins |
Drug effects |
Adipocytes |
Physiology |
Osmotic pressure |
Insulin |
Metabolism |
Monosaharid transportni proteini |
Fiziologija |
Monosaharid transportni proteini |
Učinki zdravil |
Adipociti |
Fiziologija |
Osmotski pritisk |
Inzulin |
Metabolizem |
endokrinologija |
metabolizem
Library | Call number – location, accession no. ... | Copy status |
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National and University Library, Ljubljana | GS II 523631 glavno skladišče | available - reading room |
MF, Central Medical Library, Lj. | doktorske disertacije JANEŽ Andrej Mechanisms IN: 020001414 |
available - outside loan, loan period: 1 months |
MF, Central Medical Library, Lj. | doktorske disertacije JANEŽ Andrej Mechanisms IN: 020001413 |
available - outside loan, loan period: 1 months |
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Database name | Field | Year |
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Janež, Andrej, 1971- | 18372 |
Kocijančič, Andreja, 1942-2021 | 03343 |
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