Gena za binarni toksin pri bakteriji Clostridium difficile : diplomska naloga = Binary toxin genes of bacterium Clostridium difficile : graduation thesis
Majstorović, Tamara, 1974-
Veřina sevov bakterije Clostridium difficile dela toksina TcdA in TcdB, nekateri pa tudi binarni toksin. Namen naloge je bil preiskati seve rayliřnih toksinotipov na prisotnost genov ya binarni ...toksin, gena cdtA in cdtB ter ugotoviti, ře sta gena variabilna. Pregledali smo 26 sevov 10 rayliřnih toksinotipov in 17 ribotipov. Dela genov cdtA in cdtB smo pomnoÍevali y veriÍno reakcijo s polimerayo (PCR) in namnoÍena predela BTa (375 bp) in BTb (509 bp) reyali y ustreynimi restrikcijskimi encimi. NamnoÍeni predeli pri vseh sevih se niso raylikovali niti po velikosti niti po restrikcijskih mestih. Le pri dveh sevih, IS 25 in R 9367, ki ne spadata v dosedaj ynane toksinotipe, smo dobili negativne reyultate. Gena ya binarni toksin smo naǴli pri drugih 24 sevih. Med njimi je 5 sevov, ki so bili v prejǴni rayiskavi opisani kot cdtB-negativni (M. Rupnik, ustno sporořilo). Pri teh 5 sevih trehrayliřnih toksinotipov smo primerjali reyultate dokayovanja gena cdtB dobljene y dvema rayliřnima reakcijama PCR (navadno in veřkratno PCR) na dveh rayliřnim pripravkih matriřne DNA. Ugotovili smo, da pri sevih IS 58 in R 11402 predel BTb lahko pomnoÍimo samo y navadno reakcijo PCR na řisti DNA.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [T. Majstorović] ; BF, Enota medoddelčnega študija mikrobiologije, 1999
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