Raziskave vpliva etanola na biosintezo in oksidacijo alfa-farnezena v jabolkih Granny Smith : diplomska naloga = Researching ethanol influence on biosynthesis and oxidation of alpha-farnesenein Granny Smith apples : graduation thesis
Zlatić, Emil
Ugotavljali smo spremembe vsebnosti trans, trans a-farnezena, konjugiranega trienola in spremembe aktivnosti superoksid dizmutaze, topne guaiakol peroksidaze ter koncentracije a-tokoferola in ...askorbinske kisline v koÍi jabolk 'Granny Smith'. Poskus je obsegal pet dnevno tretiranje plodov v nasiřeni atmosferi etanola in Ǵtirimeseřno skladiǴřenje tretiranih plodov pri temperaturi 0ŚC in 10ŚC. Trans, trans a-farnezen in konjugiran trienol - 3,7,11 trimetildodeka-1,3E,5E,10 tetraene-7-ol smo sintetizirali "in vitro" inju vzeli kot standard za identifikacijo in kvantifikacijo s pomořjo HPLC tehnike in 1H NMR. Aktivnosti encimov smo dolořili spektrofotometriřno z uporabo nitro blue tetrazoliuma (SOD), v primeru dolořevanja aktivnosti topnihperoksidaz smo kot substrat uporabili guaikol. Askorbinsko kislino in a-tokoferol smo dolořili s pomořjo tekořinske kromatografije visoke lořljivosti. Takoj po opravljenem tretiranju smo v plodovih skladiǴřenih na 10ŚC opazili zmanjǴanje koliřine tako askorbinske kisline kot a-tokoferola v primerjavi z kontrolo. Ugotovili smo, da pare etanola poveřajo aktivnost guaikol peroksidaze in superoksid dizmutaze. Vsebnost trans, trans a-farnezenain konjugiranega trienola je bila manjǴa v primerjavi z kontrolnim vzorcem. NiÍja temperatura skladiǴřa (0ŚC) ima enak uřinek na aktivnost omenjenih encimov. Spremembe antioksidantov so v glavnem manjǴe ali enake v primerjavi z netretiranimi plodovi. Obdelava plodov z nasiřenimi parami etanola vpliva na oksidativni stres plodov jabolk 'Granny Smith'.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [BF, Oddelek za živilstvo] : [E. Zlatič], 2000
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