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Proizvodne lastnosti seva Lactobacillus gasseri K7 pri različnih gojitvenih pogojih : diplomska naloga = Production characteristics of strain Lactobacillus gasseri K7 at different growth conditions : graduation thesisPredikaka, MarijaV predhodnih raziskavah je bilo ugotovljeno, da sev Lactobacillus gasseri K7, izolat iz blata dojenřka, tvori bakteriocine, ki zaviralno delujejo na celo vrsto bakterijskih sevov. V naǴi raziskavi ... smo prouřevali rast mikrobne populacije seva K7, porabo glukoze in tvorbo mleřne kisline ter produkcijo bakteriocinov pri razliřnih vrednostih pH gojiǴřa. Uporabili smo MRS gojiǴře, vse kultivacije so potekale pri konstantni temperaturi 37 oC. Za dolořanje bakteriocinske aktivnosti smo uporabili kvantitativno metodo za dolořanje bakteriocinov na mikrotiterskih ploǴřah, kjer smo uporabljali brezceliřni supernatant kulture. Kot indikatorski sev smo uporabili sev Lactobacillus sakei NCDO 2714. Ugotovili smo, da se bakteriocinski kompleks tvori kot primarni metabolit v logaritemski fazi rasti. Ugodni rastni pogoji niso najboljǴi za proizvodnjo bakteriocinov. Najveřjo bakteriocinsko aktivnost smo zaznali pri kultivacijah, kjer je bila rast najslabǴa, in sicer pri kultivaciji brez uravnavanja vrednosti pH (specifiřna hitrost rasti je bila 0,178/h in nastalo je 1,1 mg/ml biomase, najviǴja ugotovljena bakteriocinska aktivnost je bila 64000 BA/ml) in pri kultivacijah z uravnavanjem vrednosti pHna pH 6 (najveřja specifiřna hitrost rasti je bila 0,24/h, nastalo je 1,6 mg/ml biomase). NajviǴja dolořena bakteriocinska aktivnost pri kultivaciji z uravnavanjem vrednosti pH na pH 6 je bila 128000 BA/ml. Nevtralen pH je najmanj ugoden za tvorbo bakteriocinov, saj nismo zaznali bakteriocinske aktivnosti, rast seva pa je bila najboljǴa (nastalo je 2,0 mg/ml biomase, najveřja specifiřna hitrost rasti je bila 0,38/h). Koliřina glukoze v gojiǴřu MRS ni limitirajoři faktor pri tvorbi bakteriocinov. Zaradi relativno visoke bakteriocinske aktivnosti sta sev Lactobacillus gasseri K7 in njegov bakteriocinski kompleks zanimiva za nadaljnje raziskave.Type of material - undergraduate thesis ; adult, seriousPublication and manufacture - Ljubljana : [BF, Oddelek za živilstvo] : [M. Predikaka], 2000Language - slovenianCOBISS.SI-ID - 2470776
Author
Predikaka, Marija
Other authors
Rogelj, Irena, 1955- |
Raspor, Peter
Topics
bakteriocini |
mlečno kislinske bakterije |
Lactobacillus gasseri K7 |
mlečna kislina |
mlečna kislina |
bacteriocins |
lactic acid bacteria |
Lactobacillus gasseri K7 |
lactic acid |
lactic acid
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Library | Call number – location, accession no. ... | Copy status |
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BF, Dept. of Food Science and Technology, Lj. | Hodnik DN 974 a IN: 0012558 |
available - outside loan, loan period: 1 months |
BF, Dept. of Food Science and Technology, Lj. | Hodnik DN 974 IN: 0012557 |
available - outside loan, loan period: 1 months |
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Rogelj, Irena, 1955- | 08857 |
Raspor, Peter | 04001 |
Source: Personal bibliographies
and: SICRIS
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