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  • Molekulsko odkrivanje in sledenje bakterij rodu Pseudomonas in podskupine P. stutzeri v vzorcih iz okolja : magistrsko delo = Molecular detection and monitoring of the genus Pseudomonas and subgroup P. stutzeri in environmental samples : master of science thesis
    Stres, Blaž
    Specificity of existing primers for the specific detection of the genus Pseudomonas and P. stutzeri subgroup was evaluated in computer based analysis of 16S rRNA genes. New combinations of existing ... primers and new regions were used for preparation of optimized PCR. Specificity was experimentally determined using genomic DNA of 48 cultures belonging to the genus Pseudomonasand 10 belonging to P.stutzeri subgroup. Eleven wild type strains were isolated from soil and identified using PCR as members of the genus, eight as P. stutzeri and identified with commercial kit API 20 NE. Optimized PCR was used to develop competitive PCR (c-PCR). PCR products were analyzed using Bioanalyzer Agilent 2100 and the results obtained by regression equation. c-PCR for the specific detection of members of the genus Pseudomonasenabled us detection of 3,2 x 10 cells per gram of soil added. Methods of DNA isolation and purification used in this work provided too few target sequences to allow us specific enumeration of members of P. stutzeri subgroup with developed c-PCR. In our future work we will try to improve methods for DNA isolation and purification from soil and optimize developed c-PCR methods.
    Type of material - master's thesis ; adult, serious
    Publication and manufacture - Ljubljana : [Medicinska fakulteta] : [B. Stres], 2001
    Language - slovenian
    COBISS.SI-ID - 2506104

Library Call number – location, accession no. ... Copy status
BF, Dept. of Food Science and Technology, Lj. Knjižnica
MD 98
IN: 0012764 		available - outside loan, loan period: 1 months
MF, Central Medical Library, Lj. magistrske naloge
STRES Blaž Molekulsko
IN: 020010280 		available - outside loan, loan period: 1 months
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