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Increase in subcellular GSK-3 clusters in insulin- and adrenaline-treated differentiated rat skeletal muscle fibresFink, Katja, 1991- ...Glycogen synthase kinase 3 (GSK-3) plays an important role in m etabolic regulation in skeletal muscles, and both insulin and adrenaline stimulate GSK-3 phosphorylation . The aim of the present study ... was to study the effect of insulin and adrenaline on GSK-3 localisatio n in skeletal muscles. We characterized subcellular localization of GSK-3 signal protein in fully diffe rentiated muscle fibre by immunofluores- cence and confocal microscopy. We stimulated muscle fibres with insulin and/or adrenaline. Images were analysed by segmentation of si ngle central optical section of t he muscle. We found GSK-3 to be localised in clusters. The number of GSK-3 clusters and their average siz e were increased after stimulation with insulin and/or adrenaline. Average GSK-3 particle size is linea rly related to their quantity. We conclude that subcellular GSK-3 in isolate d skeletal muscle fibres is lo calized in clusters and clustering increased after stimulation with insulin and/or adrenaline.Source: Image analysis & stereology : official journal of the International Society for Stereology. - ISSN 1580-3139 (Vol. 39, no. 1, Mar. 2020, str. 25-32, III)Type of material - article, component part ; adult, seriousPublish date - 2020Language - englishCOBISS.SI-ID - 27393795
source: Image analysis & stereology : official journal of the International Society for Stereology. - ISSN 1580-3139 (Vol. 39, no. 1, Mar. 2020, str. 25-32, III)
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Fink, Katja, 1991- | 50628 |
Lobe Prebil, Mateja | 28325 |
Vardjan, Nina | 20214 |
Jensen, Jørgen, fizik | |
Zorec, Robert, 1958- | 03702 |
Kreft, Marko | 15666 |
Source: Personal bibliographies
and: SICRIS
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