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Izolacija eteričnih olj iz storžev, iglic in smole navadne smreke (Picea abies) in primerjava njihove sestave s plinsko kromatografijo, sklopljeno z masno spektrometrijo = Isolation of essential oils from conifer cones, leaves and resin of European spruce (Picea abies) and the comparison of their contents with gas chromatography, coupled with mass spectrometry : univerzitetni študijski program KozmetologijaDurić, InesEterična olja so hlapne, kompleksne kapljevine, katerih sinteza poteka v različnih rastlinskih organih. Navadna smreka (Picea abies), je v Sloveniji ena najpogostejših in gospodarsko najpomembnejših ... drevesnih vrst. V eteričnem olju smreke prevladujejo monoterpeni. Posebej pomembno je njihovo protimikrobno delovanje. V eksperimentalnem delu naloge smo najprej uspešno izolirali eterična olja iz oleorezina, iglic in storžev smreke. Uporabili smo metodo vodne destilacije. Kvalitativno in kvantitativno sestavo izoliranih eteričnih olj smo med seboj primerjali s plinsko kromatografijo, sklopljeno z masno spektrometrijo. Ugotovili smo, da se sestave eteričnih olj, pridobljenih iz različnih rastlinskih delov smreke, razlikujejo. S kolonsko kromatografijo smo poskušali očistiti izolirano eterično olje, a se je metoda zaradi velike hlapnosti monoterpenov izkazala kot neprimerna. Iz spojin, ki so predstavljale večinski delež naravnega eteričnega olja, smo pripravili umetno eterično olje. Čistoto referenčnih spojin smo analizirali z jedrsko magnetno resonanco. Z vonjalno analizo smo olfaktorno vrednotili naša naravna in umetno eterično olje. Večina preiskovancev je vonj eteričnih olj ocenila kot prijeten; srednje močan. Prepoznali so vonj smreke in gozda. Vonj umetnega eteričnega olja je bil podoben naravnemu. Poleg tega smo izdelali tudi preprost kozmetični izdelek, in sicerhladilni hidrogel z aromatično vodo z eteričnim oljem smreke, ki ob nanosu na kožo hladi in je prijetnega vonja. Po Evropski farmakopeji bi ga uvrstili med poltrdne dermalne farmacevtske oblike.Type of material - undergraduate thesis ; adult, seriousPublication and manufacture - Ljubljana : [I. Durić], 2019Language - slovenianCOBISS.SI-ID - 4782193
Author
Durić, Ines
Other authors
Obreza, Aleš |
Janeš, Damjan, 1977-
Topics
Eterična olja |
Kromatografija |
Spektroskopija |
eterično olje |
navadna smreka |
vodna destilacija |
analiza GC-MS |
kromatografske metode
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Library | Call number – location, accession no. ... | Copy status |
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Faculty of Pharmacy, Lj. | Knjižnica UNK 543 DURIĆ INES Izolacija 176 IN: 0016287 |
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Durić, Ines | ![]() |
Obreza, Aleš | 19061 |
Janeš, Damjan, 1977- | 28220 |
Source: Personal bibliographies
and: SICRIS
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