Identifikacija in karakterizacija EF-Tu proteina in hemadhezinov Mycoplasme synoviae = Identicfication and characterization of EF-Tu protein and hemadhesins in Mycoplasma synoviae
Narat, Mojca ...
Mycoplasma synoviae je ena od najvažnejših patogenih mikroorganizmov v intenzivni perutninarski proizvodnji. Običajna subklinična okužba dihal lahko preide v sistemsko bolezen in lahko povzroči ...vnetje sklepov. Začetek okužbe je pogojen z zmožnostjo pritrjevanja celic M. synoviae na epitelne celice dihal. To pritrjevanje omogočajo adhezini, ki sodelujejo tudi prihemadherenci in hemaglutinaciji, ki sta značilni a variabilni lastnosti. V prispevku opisujemo integralne membranske proteine pMSB in pMSA M. synoviae, (seva ULB 925), ki so povezani z variabilno hemadherenco in določajo hemaglutinacijsko pozitivne (H+) in negativne (H-) fenotipe. Spremenljivo pojavljanje teh proteinov smo dokazali z uporabo monoklonslih protiteles (mAb) in nato določili njihovo N-terminalno aminokislinsko sekvenco. Domnevamo, da pojavljanje kratkih variantnih oblik pMSB 1 proteina pri HA- fenotipu povzroča reverzibilna mutacija kot je to že znano za nekatere druge mikoplazme. V nasprotju s spremenljivimi membranskimi proteini pa se citoplazemski EF-Tu proteini izražajo konstantno. Mab R proti EF-Tu proteinu je v imunoblotih reagiral s 43 kDa proteini M. synoviae ter tudi z EF-Tu proteini 12 drugih vrst mikoplazem, ki spadajo v skupino M. hominis. Ugotovili smo N-termalno sekvenco EF-Tu proteina M. synoviae, ki ima ima v temdelu 90% identičnosti z M. hominis in 75% identičnosti z M. gallisepticum.
It was not necessary to add the material to the shelf.
Bibliographic material was successfully added on shelf.
Adding bibliographical material on shelf was not successful.
Material is already on the shelf.
Permalink
URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year
Impact factor
Edition
Category
Classification
JCR
SNIP
JCR
SNIP
JCR
SNIP
JCR
SNIP
Select the library membership card:
DRS,
in which the journal is indexed
Database name
Field
Year
Links to authors' personal bibliographies
Links to information on researchers in the SICRIS system
Subject headings in COBISS General List of Subject Headings
Select pickup location
The material from the parent unit is free. If the material is delivered to the pickup location from another unit, the library may charge you for this service.
