Morales Vasquez, Desarey; Chiem, Kevin; Ye, Chengjin; Martinez-Sobrido, Luis
Methods in molecular biology (Clifton, N.J.),
2022, Volume:
2524
Journal Article
Reporter-expressing recombinant severe acute respiratory syndrome coronavirus 2 (rSARS-CoV-2) represents an excellent tool to understand the biology of and ease studying viral infections in vitro and in vivo. The broad range of applications of reporter-expressing recombinant viruses is due to the facilitated expression of fluorescence or bioluminescence readouts. In this chapter, we describe a detailed protocol on the generation of rSARS-CoV-2 expressing Venus, mCherry, and NLuc that represents a valid surrogate to track viral infections.
