Urinary tract infection is a common infection associated with considerable societal cost and even increasing antibiotic resistance, which to some extent represents a challenging issue facing infection control. In this work, some group A Beta-lactamase genes blaTEM, bla SHV, bla CTX-M-1, bla CTX-M-2, bla CTX-M-9, bla CTX-M-25 among Uropathogenic Escherichia coli from women with cystitis have been detected. The results showed that 100 isolates of 611 urine samples belonged to Escherichia coli. Antibiotic susceptibility testing of 100 isolates to 14 antibiotics revealed that 63%, 58%, 36%, 27%, 14%, 6%, 4%, 30%, 26%, 4%, 16%, 2%, and 44% of the isolates were resistant to Ceftazidime, Cefotaxime, Piperacillin, Amoxicillin-clavulanate, Aztreonam, Piperacillin-tazobactam, Imipenem, Meropenem, Levofloxacin, Ciprofloxacin, Gentamicin, Amikacin, Nitrofurantoin, and Trimethoprome-sulfamethoxazole, respectively. The results revealed that 29% of isolates were multidrug resistant. In the current study, the results of molecular detection showed the predominance of ESBL genes in Escherichia coli isolates: blaTEM 98% followed by blaSHV 69%, and then, blaCTX-M-1 66%. blaCTX-M-9 only appeared in one isolate. Both blaCTX-M-2 and blaCTX-M-25 were not detected. The study concludes the high spreading of coexistence of more than one gene of Group A β-lactamase genes among uropathogenic Escherichia coli causes them to resist many antibiotics. This makes the treatment regimen unusual or hard to be achieved.