The research aimed to evaluate the influence exerted by the removal of the testicles (orchidectomy) in roosters on the fatty acids profile. In this regard, two batches of roosters belonging to the Transylvanian Naked Neck breed were formed; one batch was experimental (Lexp), composed of 20 birds castrated at the age of 7 weeks, and one batch was control (Lm), composed of 10 uncastrated roosters. The birds of the two groups were raised under identical conditions and received the same type of compound feed; their slaughter was performed at the age of 20 weeks. The results obtained after reporting the values of saturated fatty acids to unsaturated fatty acids recorded the highest value of 0.47 in the case of the muscles from the upper thighs of roosters (Lm). Regarding the ratio between polyunsaturated fatty acids and monounsaturated fatty acids, the highest value of 1.12 was calculated for the muscles of the upper thighs from capons (Lexp). Regarding the Ω3/Ω6 ratio, the highest value of 17.81 was calculated for the muscles of the upper thighs from the capons, while at the opposite pole was the result for the pectoral muscles of roosters from Lm, with the value of 12.48. We recommend continuing research in this direction.