This study was conducted to determine the chemical content, antibacterial, and antifungal properties of essential oil obtained from the parts of the plant called Juniperus excelsa M. Bieb. In this study, essential oils of cone, needle, and twig of J. excelsa plant, which is a naturally grown plant in Gümüşhane province, were obtained by hydrodistillation method in a Clevenger type device. Chemical composition of essential oils was determined thanks to the analysis conducted with GC-MS/FID device. Besides, antimicrobial activity tests of essential oils were decided in contrast to 23 different microorganisms with the disc diffusion method. As a result of the essential oil analysis of J. excelsa, the percentage of essential oil yield in cones, needles, and twigs was found as 5.88 %, 2.00 %, and 0.62 %, respectively. α-pinene was confirmed to be the most abundant main compound found in the essential oils of cones, needles, and twigs. As a result of the essential oil analysis of the cone, needle, and twig of J. excelsa species, it was revealed that monoterpenes were the most abundant chemical class in terms of percentage. In the antimicrobial activity test performed on the essential oils of J. excelsa plant parts, it was found that cones, needles, and twigs have a strong antimicrobial effect against Bacillus subtilis, Enterococcus faecalis, Escherichia coli, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enteritidis, Salmonella typhimurium, Staphylococcus aureus, Candida albicans, Penicillum expansum, Saccharomyces cerevisiae bacteria, yeast, and molds. Ovo je istraživanje provedeno kako bi se utvrdio kemijski sastav te antibakterijska i antifungalna svojstva eteričnog ulja dobivenoga iz dijelova biljke Juniperus excelsa M. Bieb. Za potrebe ove studije eterična ulja češera, iglica i grančica biljke J. excelsa, koja je prirodno uzgojena u pokrajini Gümüşhane, dobivena su metodom hidrodestilacije u uređaju tipa Clevenger. Kemijski sastav eteričnih ulja određen je analizom na uređaju GC-MS/FID. Osim toga, disk-difuzijskom metodom ispitano je antimikrobno djelovanje eteričnih ulja na 23 različita mikroorganizma. Analizom eteričnog ulja biljke J. excelsa utvrđeno je da je postotak prinosa eteričnog ulja u češerima, iglicama i grančicama bio (redom) 5,88 % 2,00 % i 0,62 %. Najzastupljeniji glavni spoj pronađen u eteričnim uljima češera, iglica i grančica jest α-pinen. Rezultat analize eteričnih ulja češera, iglica i grančica biljke J. excelsa pokazao je da su postotno najzastupljenija kemijska klasa monoterpeni. U ispitivanju antimikrobne aktivnosti provedenome na eteričnim uljima dijelova biljke J. excelsa ustanovljeno je da češeri, iglice i grančice imaju snažno antimikrobno djelovanje na bakterije, kvasce i plijesni Bacillus subtilis, Enterococcus faecalis, Escherichia coli, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enteritidis, Salmonella typhimurium, Staphylococcus aureus, Candida albicans, Penicillum expansum i Saccharomyces cerevisiae.