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Wilson, Catherine R; Mitchelmore, Philip J; Withers, Nicholas; Brown, Alan R
Diagnostic microbiology and infectious disease, September 2024, Volume: 110, Issue: 1Journal Article
•H. influenzae strains can be identified from sputum using culture-independent MLST.•Culture-independent MLST can be used to screen for H. influenzae cross-infection.•Using MLST, there was no evidence of cross-infection of H. influenzae in non-CF bronchiectasis. Whether cross-infection of respiratory pathogens between patients with non-cystic fibrosis bronchiectasis occurs is debated. Investigation with traditional microbiological culture risks simplifying the lung microbiome. We demonstrate the use of culture-independent Multilocus sequence typing to screen for Haemophilus influenzae strain types in a cohort of twenty-eight patients with non-cystic fibrosis bronchiectasis.
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