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Parodi, M T; Baffico, M; Tonini, G P; Dini, G; Ferrea, G; Massimo, L; Cornaglia-Ferraris, P
International journal of cell cloning, 07/1986, Volume: 4, Issue: 4Journal Article
A long-term culture of bone marrow lymphoblasts in a case of unclassified acute lymphoblastic leukemia is described. Cells lacking any lymphocytic marker in the early phase of the culture were gradually substituted by B cells showing a pattern of polyclonality. The culture supernatant contained high levels of immunoglobulins also showing interleukin 2 activity. Search for antigens related to the Epstein-Barr virus was negative. A clonal expansion of B cells versus spontaneous differentiation of unclassified leukemic cells is discussed; the long-term culture technique as a tool for a better evaluation of leukemic cells is suggested and discussed.
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