Due to constant exposure to high torque and loads toughness, compressive durability and light weight of joint parts are crucial factors for biped walking humanoid robot parts. When polymer transmission parts are used for the manufacture of humanoid robot joint, wear becomes an important factor in terms of transmission error (backlash) between the input and output axes, ex. between the motor and the robot’s leg joint. In such joint system, a polymer bushes is directly connected to a cam plate, playing an important role in the robot's movement ability. In this work, the influence of wear of reinforced poly-ether-ether-ketone (PEEK) polymer bushes in friction against 7075 aluminium alloy cam plates is investigated in order to establish the application possibilities in transmission parts in humanoid robot’s joint. The PEEK bush surface conditions as well as the input axis-output axis backlash require close examination, so that efficient systems can be built. Sliding wear tests were performed on bushes under 0-50kgfcm loaded torque while the cam plate oscillated. Based on the wear observation, it was found that in a high load range the backlash increased along with number of cycles due to PEEK bush wear. The bush surface roughness also increased during testing. Roughness of PEEK bush surface and the loaded torque for output axis were significantly related to backlash of output axis in robot joint.