E-resources
-
陈璇;刘海霞;彭显;邹玲
华西口腔医学杂志, 2017, Volume: 35, Issue: 6Journal Article
目的利用框内缺失法,通过IFDC2基因盒及融合聚合酶链反应(PCR)、同源重组技术构建变异链球菌UA159菌株srtA基因缺失株。方法PCR扩增变异链球菌UA159菌株srtA基因上下游同源片段及IFDC2基因盒,将这些片段连接、转化入UA159,替换srtA基因同源片段,筛选、鉴定红霉素抗性克隆;PCR扩增、连接UA159srtA基因上下游同源片段,将连接片段转化人前述红霉素抗性克隆中替换IFDC2同源片段,筛选、鉴定抗苯丙氨酸类似物p-chloro-phenylalanine(p-C1-Phe)克隆。结果PCR、琼脂糖凝胶电泳及DNA测序结果均证明srtA基因编码区被完全删除,上下游片段无缝连接,成功构建UA159 srtA基因缺失株。结论本研究成功构建了无标记的变异链球菌UA159srtA基因缺失株,为进一步研究该基因在生物膜中的作用及其调控机制奠定了基础。
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
If the library membership card is not in the list,
add a new one.
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|
Source: Personal bibliographies
and: SICRIS
The material is available in full text. If you wish to order the material anyway, click the Continue button.