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Teng, Geer; Wang, Qianqian; Cui, Xutai; Wei, Kai; Xiangli, Wenting; Chen, Guoyan
Journal of Raman spectroscopy, April 2021, Volume: 52, Issue: 4Journal Article
In clinical field, the diagnosis of many diseases and their development stages depend on the detection of the corresponding bacteria. Raman spectroscopy and laser‐induced breakdown spectroscopy (LIBS) are two novel spectral diagnostic technologies for clinical bacteria identification. Both of them have been used in clinical detection combined with optimized support vector machine (SVM). In this paper, two feature‐level fusion methods (before feature selection fusion BFSF and after feature selection fusion AFSF) were proposed to improve the performance of SVM classifier and reduce the analyzing time (including the parameter tuning, model training, and testing time) simultaneously by combining data of LIBS and Raman spectroscopy. Using the most important 10 feature lines as the inputs of the optimized classifier, the analyzing time could be reduced to 1 to 2 min and the correct classification rate (CCR) achieved 95.67%. Without optimizing SVM parameters, the two proposed methods could achieve rapid and accurate classification of pathogenic bacteria further. The AFSF method showed better results with less fusion features while the BFSF method achieved higher CCR at 100% with more features. Both two methods costed around 0.2 s for analysis. These indicate that the proposed feature‐level fusion methods can improve the performance of SVM for bacteria detection. Maintaining the highest diagnostic accuracy, they can achieve the minimum analyzing time. In this paper, we proposed two feature‐level fusion methods (before feature selection fusion BFSF and after feature selection fusion AFSF) to improve the performance of SVM classifier and reduce the analyzing time simultaneously by combining data of LIBS and Raman spectroscopy. AFSF method showed better results with less fusion features while the BFSF method achieved higher accuracy at 100% with more features. The proposed feature‐level fusion methods can achieve LIBS‐Raman diagnosis around 0.2 s for bacteria.
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