E-resources
-
Berthet, Virginie; Rigot, Véronique; Champion, Serge; Secchi, Jeannine; Fouchier, Francis; Marvaldi, Jacques; Luis, José
The Journal of biological chemistry, 10/2000, Volume: 275, Issue: 43Journal Article
Some integrin α subunits undergo a post-translational cleavage in their extracellular domain. However, the role of this cleavage in integrin function is unclear. Enzymes involved in this maturation belong to the subtilisin-like endoprotease family (convertases). To understand the role of the α subunit cleavage in integrin function, we have designed stable transfectants (PDX39P cells) expressing α1-PDX, a convertase inhibitor. Immunoprecipitation of cell surface proteins from PDX39P showed that α3, α6 and αvintegrins lack endoproteolytic cleavage. We have compared adhesion between PDX39P cells and mock-transfected cells on different extracellular matrix proteins. No difference in adhesion could be observed on laminin-1 and type I collagen, while attachment of PDX39P cells to vitronectin (ligand of the αvβ5integrin) was dramatically reduced. The reduced adhesion of PDX39P cells was not due to changes in integrin affinity as determined by solid-phase receptor assay in a cell-free environment. Intracellular signaling pathways activated by αv integrin ligation were also affected in PDX39P cells. It thus seems that the absence of endoproteolytic cleavage of αv integrins has important consequences on signal transduction pathways leading to alterations in integrin function such as cell adhesion.
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
If the library membership card is not in the list,
add a new one.
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|
Source: Personal bibliographies
and: SICRIS
The material is available in full text. If you wish to order the material anyway, click the Continue button.