Background: Premature atrial contractions (PACs) are predictors of atrial fibrillation, stroke, and cardiovascular mortality. The present study aimed to assess relevant factors for PACs among a general population of Japanese men.Methods and Results: This study conducted a population-based, cross-sectional study among 517 men, aged 40–79 years, with neither apparent myocardial infarction nor atrial fibrillation. 24-h Holter electrocardiography to assess PAC frequency was used. Age, body mass index, height, low-density lipoprotein cholesterol, triglycerides, high-density lipoprotein cholesterol, mean heart rate, diabetes mellitus, hypertension, physical activity, smoking, alcohol consumption, and lipid-lowering therapy were included in multivariable negative binomial regression analyses to assess correlation for the number of PACs per hour. Almost all participants (99%) had at least 1 PAC in 1 h (median number 2.84 PACs per h). In multivariable negative binomial regression after adjusting for all covariates simultaneously, age (relative risk 95% confidence interval, 1.30 1.08–1.57 per 1-standard deviation SD increment), height (1.19 1.02–1.39 per 1-SD increment), triglycerides (0.79 0.65–0.97 per 1-SD increment), mean heart rate (0.69 0.59–0.80 per 1-SD increment), physical activity (0.63 0.43–0.93), current smoking (1.69 1.06–2.69), current moderate (1.97 1.23–3.16) and heavy (1.84 1.12–3.01) alcohol consumption were independently associated with PAC frequency.Conclusions: PAC frequency was independently associated with age, height, smoking, alcohol consumption, heart rate, physical activity, and triglycerides.