Anaerobic digestion (AD) converts biomass to biogas. However, its performance is often affected by the nutrient condition of AD substrate. In this study, a few substrate supplements were selected to promote the biogas production; MgO, FeCl 3 , and cellulase were selected based on the result from elemental analyses of the biomass. The potential impact of the additives on AD process was evaluated by performing a series of biochemical methane potential (BMP) tests. BMP reactors with the substrate with one of the selected additives (i.e., MgO of 380 mg Mg L −1 , FeCl 3 of 88 mg Fe L −1 or cellulase of 25 mg L −1 ) exhibited higher microbial activity; 5–15% more biogas production was observed, compared to the blank. Microbial community analysis showed that different additives resulted in proliferation of different microbial species. Therefore, it was decided to add the mixture of the three additives to the biomass. Addition of the mixed additive resulted in 22% more gas production.