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Goodwin, Michael P.; Grinfeld, Dmitry; Yip, Ping; Bowen, Kyle P.; Kafader, Jared O.; Kelleher, Neil L.; Senko, Michael W.
Journal of the American Society for Mass Spectrometry, 04/2024, Volume: 35, Issue: 4Journal Article
The quality of data in charge detection mass spectrometry depends on accurate determination of ion charge. While the method of selective temporal overview of resonant ions (STORI) has proven to be highly enabling for determining the charge of ions that survive for variable amounts of time, it assumes that the ion frequency exactly matches the frequency being used in the calculation. Any mismatches result in low charge estimates. To address this, the misSTORI method was developed to correct these discrepancies. This can significantly reduce the charge measurement errors for samples with unstable masses. As an example, the misSTORI approach can eliminate a 5.7% charge determination error for a VP3-only AAV capsid that shifts 25 ppm in mass.
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