We have investigated characteristics of ion upflow and naturally enhanced ion‐acoustic lines (NEIALs) based on the European Incoherent Scatter (EISCAT) Svalbard radar (ESR) data continuously obtained between March 2007 and February 2008. For the ion upflow study we have used approximately 78,000 field‐aligned profiles obtained with the ESR. For the NEIAL study we have identified approximately 1500 NEIALs in the ESR data at altitudes between 100 and 500 km. The occurrence frequency of ion upflow shows two peaks, at about 0800 and 1300 magnetic local time (MLT), while only one strong peak is seen around 0900 MLT for NEIALs. The upward ion flux also has only one peak around 1100–1300 MLT. The occurrence frequency of ion upflow varies strongly over season. It is higher in winter than in summer, whereas NEIALs are more frequent in summer than in winter. NEIALs frequently occur under high geomagnetic activity and also high solar activity conditions. Approximately 10% of NEIALs in the F region ionosphere were accompanied by NEIALs in the E region (occurred at altitudes below 200 km). About half of the E region enhanced echoes did not have an F region counterpart. Upshifted NEIALs dominate in the E region whereas downshifted NEIALs are usually stronger above an altitude of 300 km. The high occurrence frequency of NEIALs in the prenoon region (0800–1000 MLT) might be associated with acceleration of thermal ions to suprathermal ones. At the same MLT and geomagnetic latitude suprathermal ions and broadband extremely low frequency (BBELF) wave activity have been observed, according to previous studies.