E-resources
-
Cassman, Marvin; Englard, Sasha
The Journal of biological chemistry, 02/1966, Volume: 241, Issue: 4Journal Article
The stoichiometry of reduced diphosphopyridine nucleotide binding to supernatant malic dehydrogenase (S-MDH) was determined by fluorometric techniques and a Sephadex gel filtration procedure. Both types of measurement yielded a stoichiometry of 1 mole of DPNH bound per mole of enzyme. This stoichiometry was unaffected by the presence of d -malate, a compound which is known to form a ternary enzyme complex having distinctive fluorescent properties. The dissociation constants for the binary enzyme-DPNH and ternary enzyme-DPNH- d -malate complexes with respect to DPNH were determined. Several criteria were used to determine the effectiveness of a number of compounds in forming ternary complexes with S-MDH and DPNH. A requisite for ternary complex formation was a dicarboxylic acid with chain length of 3 to 4 carbon atoms. Although an α-hydroxyl group was not necessary for ternary complex formation, the introduction of such a group in a specific steric configuration resulted in a loss of capacity to form such a complex. Formation of strong fluorescing complexes by a number of dicarboxylic acids is not necessarily related to their effectiveness as inhibitors of S-MDH activity. The presence of an α-hydroxyl group appears to be required for inhibition, although added steric considerations involving the β carbon atom may obliterate such inhibitory capacity.
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
If the library membership card is not in the list,
add a new one.
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|
Source: Personal bibliographies
and: SICRIS
The material is available in full text. If you wish to order the material anyway, click the Continue button.