In the Muon g-2 Experiment at Fermilab, a calibration apparatus based on a set of laser sources and a distribution system has been designed and implemented by the INFN group. The light pulses are read by specific photo-detectors, whose signals are digitized by custom electronics modules designed to match the experimental requirements. The data frames of each module are transmitted to a controller board that performs the event-building process and transfers the reconstructed data to the online farm. In this work we present the architecture and data flow of the acquisition system that depends on the laser calibration program defined inside the Laser Control board. Experimental results on the overall system performances, also including the software processes running both at controller and farm level will be described. •A custom DAQ system performs a trigger-driven data collection.•Each crate hosts a master board and several slaves based on FPGA elements.•The crate event building is fully realized in hardware.•Monitoring facility to track in realtime data transfers and processing activities.