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Sepetdjian, Elizabeth; Shihadeh, Alan; Saliba, Najat A.
Food and chemical toxicology, 05/2008, Volume: 46, Issue: 5Journal Article
An analytical method for the determination of 16 polycyclic aromatic hydrocarbons (PAHs) in the mainstream of narghile smoke is presented. The smoke was generated using a digital waterpipe smoking machine connected to the mouthpiece of a narghile that was loaded with 10g of a popular flavored tobacco and kept alight with quick-light charcoal briquettes that are commonly used for this purpose. A standard smoking regimen consisting of 171 puffs of 530ml volume and 2.6s duration spaced 17s apart was used, and the smoke condensates were collected on glass fiber filters. PAHs were extracted with toluene assisted by sonication. For purification, the extract was passed through a silica cartridge and eluted with hexane. The eluent was preconcentrated, reconstituted in acetonitrile, and analyzed using a GC–MS–SICP method. The method showed good selectivity, repeatability, accuracy and sensitivity. The limit of detection ranged from 15 to 96ng for benzoapyrene and indeno1,2,3-cdpyrene, respectively. It was found that a single narghile smoking session delivers approximately 50 times the quantities of carcinogenic 4- and 5-membered ring PAHs as a single 1R4F cigarette smoked using the FTC protocol. The pattern of PAH concentrations suggested that formation pathways differ from those of the cigarette, possibly reflecting the differing combustion conditions of the two smoking devices.
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