Here we describe fossil vertebrate remains from a new Solimões Formation (Eocene-Pliocene) locality, near the highway BR 364 at Sena Madureira municipality (Acre, Brazil). The findings comprise more than forty-four isolated fragments from which twenty-three specimens could be properly identified anatomically and systematically. The materials include at least three different Caimaninae genera (Caiman, Melanosuchus, and Mourasuchus), two distinct side-necked turtles (Chelus and Podocnemididae) and one mammal (Toxodontidae). The studied fossils could be systematized into two taphonomic classes based on their preservational status, indicating a sinuous fluvial meandering paleoenvironment. This new locality seems to be relevant regarding not only its fossil vertebrate diversity but also because of its geographic position: on a highway side, which allows access at any season of the year, as contrary to most common riverine outcrops from Solimões Formation, which are restricted to collections during the dry seasons. •New outcrop is described with several fossil vertebrate remains.•Increase of knowledge regarding Solimões Formation in the interfluvial regions.•Rich fossil tetrapod assemblage, presenting turtles, notoungulates, and crocodiles.•Description of the first postcranial Melanosuchus fossil from Solimões Formation.•Fossils systematized in taphonomical classes according to preservational patterns.