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Saer, Rafael G.; Stadnytskyi, Valentyn; Magdaong, Nikki C.; Goodson, Carrie; Savikhin, Sergei; Blankenship, Robert E.
Biochimica et biophysica acta. Bioenergetics, April 2017, 2017-Apr, 2017-04-00, 2017-04-01, Volume: 1858, Issue: 4Journal Article
In this paper we report the steady-state optical properties of a series of site-directed mutants in the Fenna-Matthews-Olson (FMO) complex of Chlorobaculum tepidum, a photosynthetic green sulfur bacterium. The FMO antenna complex has historically been used as a model system for energy transfer due to the water-soluble nature of the protein, its stability at room temperature, as well as the availability of high-resolution structural data. Eight FMO mutants were constructed with changes in the environment of each of the bacteriochlorophyll a pigments found within each monomer of the homotrimeric FMO complex. Our results reveal multiple changes in low temperature absorption, as well as room temperature CD in each mutant compared to the wild-type FMO complex. These datasets were subsequently used to model the site energies of each pigment in the FMO complex by employing three different Hamiltonians from the literature. This enabled a basic approximation of the site energy shifts imparted on each pigment by the changed amino acid residue. These simulations suggest that, while the three Hamiltonians used in this work provide good fits to the wild-type FMO absorption spectrum, further efforts are required to obtain good fits to the mutant minus wild-type absorption difference spectra. This demonstrates that the use of FMO mutants can be a valuable tool to refine and iterate the current models of energy transfer in this system. Display omitted •The environment of each pigment in the FMO complex was perturbed by mutagenesis.•Each FMO mutant alters the optical properties of the complex in a specific manner.•Datasets obtained from the mutants were simulated with three different Hamiltonians.•Simulations of the mutant spectra fit the absorption data better than CD data.•Data from FMO mutants can be useful tools for future biophysical simulations.
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