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Sun, Rui-cong; Hu, Jia-huan; Li, Xiao-han; Liu, Chun-chun; Liu, Ya-yun; Chen, Jing; Yang, Yi-chen; Zhou, Bin
Veterinary microbiology, September 2022, 2022-09-00, 20220901, Volume: 272Journal Article
Classical swine fever virus (CSFV), a member of the Flaviviridae enveloped RNA virus family, results in an epidemic disease that brings serious economic losses to the pig industry worldwide. Valosin-containing protein (VCP/p97), a multifunctional active protein in cells, is related to the life activities of many viruses. However, the role of VCP in CSFV infection remains unknown. In this study, it was first found that treatment of VCP inhibitors impaired CSFV propagation. Furthermore, overexpression or knockdown of VCP showed that it was essential for CSFV infection. Moreover, confocal microscopy and immunoprecipitation assay showed that VCP was recruited for intracellular transport from early endosomes to lysosomes. Importantly, knockdown of VCP prevented CSFV to release from early endosomes, suggesting that VCP is a key factor for CSFV trafficking. Taken together, our findings first demonstrate that the endocytosis of CSFV into PK-15 cells requires the participation of VCP, providing the alternative approach for the discovery of novel anti-flaviviridae drugs. •This is the first report that VCP inhibits CSFV infection.•In the early stage of infection, VCP is involved in CSFV endocytosis.•VCP colocalizes and interacts with Rab5 and Lamp-1 after CSFV entry.•CSFV is restricted to early endosomes upon VCP depletion.
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