Rapid improvement of grain weight via highly efficient CRISPR/Cas9-mediated multiplex genome editing in rice

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Rapid improvement of grain weight via highly efficient CRISPR/Cas9-mediated multiplex genome editing in rice

Xu, Rongfang; Yang, Yachun; Qin, Ruiying; Li, Hao; Qiu, Chunhong; Li, Li; Wei, Pengcheng; Yang, Jianbo

Journal of genetics and genomics, 08/2016, Volume: 43, Issue: 8

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Most of the important agronomic traits in crop plants, such as yield, quality and stress response, are quantitative and jointly controlled by many genomic loci or major genes. Improving these complex traits depends on the combination of beneficial alleles at the quantitative trait loci （QTLs）. However, the conventional cross breeding method is extremely time-consuming and laborious for pyramiding multiple QTLs. In certain cases, this approach might be technically difficult because of close linkage between genes separately responsible for desirable and undesirable traits.

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