E-resources
Peer reviewed
Open access
-
Watermann, Christian; Pasternack, Helen; Idel, Christian; Ribbat-Idel, Julika; Brägelmann, Johannes; Kuppler, Patrick; Offermann, Anne; Jonigk, Danny; Kühnel, Mark Philipp; Schröck, Andreas; Dreyer, Eva; Rosero, Christian; Nathansen, Jacqueline; Dubrovska, Anna; Tharun, Lars; Kirfel, Jutta; Wollenberg, Barbara; Perner, Sven; Krupar, Rosemarie
Clinical cancer research, 01/2021, Volume: 27, Issue: 2Journal Article
Recurrent tumors (RT) of head and neck squamous cell carcinoma (HNSCC) occur in up to 60%, with poor therapeutic response and detrimental prognosis. We hypothesized that HNSCC RTs successfully evade antitumor immune response and aimed to reveal tumor immune microenvironment (TIME) changes of primary tumors (PT) and corresponding RTs. Tumor-infiltrating leukocytes (TIL) of 300 PTs and 108 RTs from two large independent and clinically well-characterized HNSCC cohorts discovery cohort (DC), validation cohort (VD) were compared by IHC. mRNA expression analysis of 730 immune-related genes was performed for 18 PTs and RTs after adjuvant chemoradiotherapy (CRT). The effect of chemotherapy and radiation resistance was assessed with an spheroid/immunocyte coculture model. TIME analysis revealed overall decrease of TILs with significant loss of CD8 T cells (DC = 0.045/VC < 0.0001) and B lymphocytes (DC = 0.036/VC < 0.0001) in RTs compared with PTs in both cohorts. Decrease predominantly occurred in RTs after CRT. Gene expression analysis confirmed loss of TILs ( = 0.0004) and B lymphocytes ( < 0.0001) and showed relative increase of neutrophils ( = 0.018), macrophages ( < 0.0001), dendritic cells ( = 0.0002), and mast cells ( = 0.0057) as well as lower overall expression of immune-related genes ( = 0.018) in RTs after CRT. Genes involved in B-lymphocyte functions and number of tertiary lymphoid structures showed the strongest decrease. and were upregulated and , indicating their potential suitability as therapeutic targets in CRT resistance. HNSCC RTs have an immunosuppressive TIME, which is particularly apparent after adjuvant CRT and might substantially contribute to poor therapeutic response and prognosis.
Author
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
If the library membership card is not in the list,
add a new one.
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|
Source: Personal bibliographies
and: SICRIS
The material is available in full text. If you wish to order the material anyway, click the Continue button.