Nitrogen fertilization is one of the common farming practices in vine production which may affect the increase in vegetative mass of vine and grape yield, but also the dynamics of maturation related primaryly to differences in the concentrations of sugar, total acidity, and content of organic acids in grapes. The experiment was conducted in 2006 – 2007 years on cultivars Chardonnay, Riesling and Italian Riesling, with three different intensities of nitrogen fertilization 23 kg, 70 kg and 117 kg N/ha. The concentrations of sugar, malic and citric acid have not been changed due to nitrogen fertilization. Differences were observed in the Riesling grapes from 2006th year with 23 kg and 70 kg N/ha fertilization, and in the Chardonnay grapes with 117 kg N / ha fertilization.