The problem of the current paper arises from the individual’s need to hold himself accountable and reconsider his behaviors or actions when many situations passing him in daily in which he lives and keeps into contact with those around him. This situation is accompanied by either psychological pain or the individual’s feeling of relief, each according to the nature of the personality of this individual and his emotional state. The individual’s feeling of guilt is nothing but a necessity for the individual to discipline himself and address the mistakes and abusive actions in order to stop them once and for all, provided that the situation does not reach the feeling of imaginary guilt, which is considered obstructing his thinking and leads him to give errors greater than their normal size, which is what happens in patients having depression. Al-Ansari’s study (2001) maintained that university students suffer from situational guilt at a rate of (8.9%), while the percentage of university female students reached (17.4%). This gives an indication that female students are more likely to feel situational guilt than male students and that these percentages are serious and cannot be underestimated. Because the kindergarten students are part of the university students, their role as future teachers for an important segment of society is the focus, namely Kindergarten Dept. The kindergarten teacher should be more responsible and more psychologically and emotionally balanced in its dealings with the different situations that it brings together with the child and be alert to the conscience to achieve equality between children, taking into account the individual differences between them, so the problem of the current research was identified by the following question: Is there a relationship between situational guilt and conscientious awareness for kindergarten students? The current research aims to identify the relationship between situational guilt and conscientious awareness among female kindergarten students. The research sample consisted of (300) female students from the Kindergarten Department, and to achieve the goal of the research, two scales were prepared: the Situational Guilt Scale and the Conscientious Awareness Scale for the Kindergarten Department students. Through reviewing the literature and previous studies, the researcher identified (32) items for the Situational Guilt Scale and (24) to measure the final form of conscientious awareness, the validity, and reliability of the two scales were confirmed. After using the statistical methods, the research reached the following results: • The research sample has a high degree of situational guilt. • The research sample has a high degree of conscientious awareness. • There is a high correlation between situational guilt and conscientious awareness among female students of the Kindergarten Department.