Ramirez et al discuss the enrollment to date in the All of Us Research Program, a cohort study launched in 2018 designed to enroll >1 million participants, including those from historically underrepresented backgrounds, to diversify the human genome database and the infrastructure it is building to broadly share its data assets to improve and accelerate biomedical research. The All of Us Research Program is a longitudinal cohort initiative aimed at advancing precision medicine and improving human health by enrolling at least 1 million diverse individuals across the US. The program was created following the announcement of the Precision Medicine Initiative in 2015, began national enrollment in 2018, and is funded through fiscal year 2026 with approximately $1.5 billion appropriated in the 21st Century Cures Act.