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Oliveira, Renato C. de(Faculdade Assis Guagacz Departamento de Ciências Biológicas); Neves, Pedro M.O.J.(Universidade Estadual de Londrina Centro de Ciências Agrárias Departamento de Agronomia); Alves, Luís F.A.(Universidade Estadual do Oeste do Paraná CCBS Laboratório de Zoologia)
Neotropical entomology, 2004, Volume: 33, Issue: 3Journal Article
A virulência de isolados dos fungos entomopatogênicos Beauveria bassiana, Metarhizium anisopliae e Paecilomyces fumosoroseus foi avaliada sobre Oligonychus yothersi (McGregor) em laboratório. Os bioensaios de seleção e de determinação de CL50 e TL50 foram realizados com discos foliares de erva-mate previamente infestados com 10 fêmeas adultas do ácaro. Para cada isolado um conjunto de cinco discos foram pulverizado com 1 ml da suspensão padronizada em 1,0x10(7) conídios/ml (50 ácaros por tratamento). Após pulverizados os discos foram mantidos flutuando em água destilada em caixas plásticas (3 cm diâmetro e 1,5 cm altura), em câmara tipo B.O.D. (temperatura: 25 ± 1ºC; UR: 70 ± 10% e fotofase de 12h). A mortalidade total foi avaliada cinco dias após a inoculação. Os ácaros mortos foram transferidos para câmara úmida para confirmação de mortalidade, sendo examinados sob microscópio ocular seis dias após a morte. O fungo B. bassiana apresentou grande potencial como agente de controle microbiano, podendo ser incorporado em programas de manejo integrado do ácaro vermelho da erva-mate, O. yothersi. Os isolados de B. bassiana UEL02, UEL08, UEL10, UEL50, CG082, CG166, CG375, CG424 e CG481 foram os mais virulentos. Eles apresentaram mortalidades corrigida e confirmada superiores a 70% e valores estimados de CL50 variando entre 1,9x10(6) e 6,0x10(7) conídios/ml e de TL50 variando entre 3,3 e 4,3 dias. The virulence of strains of the entomopathogenic fungi Beauveria bassiana, Metarhizium anisopliae and Paecilomyces fumosoroseus was evaluated on Oligonychus yothersi (McGregor) in laboratory. The bioassays for strain selection and determination of LD50 and LT50 were elaborated with leaf disks of Paraguay tea infested with 10 adult females of the mite. Groups of five disks were sprayed with 1 ml of the suspension standardized in 1.0x10(7) conidia per ml (50 mites per treatment) of each strain. After that, the disks were maintained floating in distilled water in plastic boxes (3 cm diameter and 1.5 cm height), in environmental chamber (temperature: 25 ± 1ºC; RH: 71 ± 10% and 12h photophase). Five days after the inoculation the total mortality was evaluated, and the dead mites were transferred to humid chamber. Six days after death, the sporulation in the cadavers was examined under an optical microscope. B. bassiana presented great potential as a microbial control agent, and can be incorporated in integrated pest management of the Paraguay tea red mite, O. yothersi. The B. bassiana strains UEL02, UEL08, UEL10, UEL50, CG082, CG166, CG375, CG424 and CG481, were the most virulent, with corrected and confirmed mortality higher than 70% and estimated value of CL50 varying between 1.9x10(6) and 6.0x10(7) conidia per ml and TL50 varying between 3.3 and 4.3 days.
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