Gomes, M, Fitas, A, Santos, P, Pezarat-Correia, P, and Mendonca, GV. Validation of a single session protocol to determine the load-velocity profile and one-repetition maximum for the back squat exercise. J Strength Cond Res 38(6): 1013-1018, 2024-We investigated whether a single session of absolute incremental loading is valid to obtain the individual load-velocity profile (LVP) and 1 repetition maximum (1RM) for the free-weight parallel back squat. Twenty strength-trained male subjects completed 3 testing sessions, including a baseline 1RM session and 2 LVP sessions (LVP rel based on incremental relative loads and LVP abs based on absolute load increments until 1RM). The 1RM load was compared between the baseline and LVP abs . The load at zero velocity (load-axis intercept L 0 ), maximal velocity capacity (velocity-axis intercept V 0 ), slope, and area under the load-velocity relationship line (A line ) were compared between the LVP rel and LVP abs using equivalence testing through 2 one-sided t -tests. Measurement accuracy was calculated using the absolute percent error. The 1RM measured at baseline and LVP abs was equivalent and presented a low absolute percent error (1.2%). The following LVP parameters were equivalent between LVP rel and LVP abs : 1RM, L 0 , and A line because the mean difference between sessions was close to zero and the Bland-Altman limits of agreement (1RM:5.3 kg; L 0 :6.8 kg; A line : 9.5 kg·m -1 ·s -1 ) were contained within the a priori defined ± equivalent margins (5% for 1RM and L 0 and 10% for A line ). The aforementioned variables presented a low absolute percent error. However, slope and V 0 were not equivalent between sessions. In conclusion, a single session of absolute incremental loading is a valid approach to obtain the L 0 and A line of the individual LVP and 1RM, and can be used to efficiently track the magnitude of neuromuscular adaptations throughout the training cycles for the free-weight back squat.