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杨锡金; 王艳; 陈梓瀚; 刘明芸; 吴俊; 向杨; 陈荣祥; 周亚平
食品工业科技, 08/2023, Volume: 44, Issue: 16Journal Article
R917; 目的:基于谱效关系研究,寻找景天三七抗氧化活性的物质基础,为其质量控制提供一定的参考依据.方法:建立不同产地景天三七的 HPLC-ECD(High performance liquid chromatography-Electrochemical Detection,HPLC-ECD)指纹图谱并进行相似度评价;通过偏最小二乘回归分析和灰色关联分析研究指纹图谱与其抗氧化活性之间的谱效关系.结果:建立了景天三七的HPLC-ECD指纹图谱,样品间的相似度较高,共标定 17个共有峰,通过与对照品比对鉴别出了没食子酸、原儿茶酸、色氨酸、没食子酸甲酯、表没食子儿茶素、咖啡酸、表没食子儿茶素没食子酸酯、鸢尾酚酮、杨梅苷、异槲皮苷、迷迭香酸和槲皮苷等 12种成分;谱效关系表明,没食子酸、原儿茶酸、表没食子儿茶素、表没食子儿茶素没食子酸酯、迷迭香酸和槲皮苷的VIP值均大于 1;其中没食子酸、迷迭香酸和槲皮苷的关联度接近于 0.8.分子对接结果表明,表没食子儿茶素没食子酸酯、杨梅苷、异槲皮苷、迷迭香酸和槲皮苷的Libdock Score较高.因此,表没食子儿茶素没食子酸酯、杨梅苷、异槲皮苷、迷迭香酸和槲皮苷等化合物为景天三七抗氧化活性的关键成分.结论:结合化学计量学方法进行谱效关系研究,确定了景天三七抗氧化活性物质基础,可为景天三七的质量评价提供了一定的科学依据.
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