This paper presents the results of soil research in a mycological reserve that is defined as a special nature reserve according to the IUCN categorization. Ecologically, fungi are the most valuable element in the biological diversity of protected reserves. Favorable climate and the complexity of geology, soils and vegetation of Lisina Mountain caused the development of extraordinarily rich mycoflora. The soil cover here is characterized mainly by soils on acid siliceous parent rocks. Carbonate sediments as a part of volcanic sediment formation, present with laminated black limestone, complicate the structure of the soil cover. The protected area is defined by five basic soil types: rendzina, ranker, dystric cambisols, luvisol and podzol. Soil properties depend on pedogenetic processes and the nature of the parent rock. Rendzina is the most common soil type on limestone-dolomite substrate, where it creates elementary and complex soil combinations, mainly because of its strong relief. The most common soil type on acid silicate rocks is dystric cambisols. nema