Objectives: Diabetes mellitus is a chronic, lifelong condition that affects our body physiology. Untreated diabetes mellitus causes diseases such as diabetic retinopathy, diabetic nephropathy and diabetic neuropathy, auto immune diseases, polyuria, polydipsia, loss of weight, and cardiovascular diseases. The use of medications for the treatment of diabetes mellitus causes adverse effects with long-term use, and sometimes leads to death. Today, researchers are working on the discovery of new anti-diabetes drugs from plants with low or no adverse effects. From this point of view, the present work was conducted to evaluate the anti-diabetic activity of Zornia gibbosa Span.Materials and Methods: This acute toxicity study was conducted for ethyl acetate and ethanol (70%v/v) extracts of Z. gibbosa as per OECD guidelines. The anti-diabetic activity of selected plant extracts were tested using alloxan-induced diabetes in a rat model.Results: No mortality was observed in the administered doses of Zornia gibbosa Span extracts. The tested extracts significantly (pless than or equal to0.01) restored the physiologic changes that occurred due to the alloxan-induced diabetes mellitus. The hydroalcoholic extracts at 500 mg/kg body weight concentration showed more activity compared with other extracts at different concentrations along with standard drug (glibenclamide). Zornia gibbosa significantly decreased glucose concentrations and restored the altered enzymes levels caused by damage to different organs by diabetes.Conclusion: The results of the present study indicate that Z. gibbosa has a significant anti-diabetic activity. Therefore, it may be capable of use as an alternate medicine along with allopathic medicine in the treatment of diabetes as well as its health problems.Key words: Zornia gibbosa Span, diabetes mellitus, alloxan, glibenclamideAmac: Diyabet, vucudumuzun fizyolojisini etkileyen, yasam boyu kronik bir hastaliktir. Tedavi edilmeyen diabetes mellitus, diyabetik retinopati, diyabetik nefropati ve diyabetik noropati, otoimmun hastaliklar, ploiuri, polidipsi, kilo kaybi ve kardiyovaskuler hastaliklar gibi hastaliklara neden olur. Diabetes mellitus tedavisinde kullanilan ilaclar, uzun sureli kullanimda yan etkilere neden olur, bazen olume yol acar. Gunumuzde arastirmacilar, yan etkileri dusuk veya hic olmayan bitkilerden hareketle yeni anti-diyabetik ilaclarin kesfedilmesi uzerinde calismaktadir. Bu acidan, mevcut calismada, Zornia gibbosa Span'in anti-diyabetik etkinliginin degerlendirilmesi amaclanmistir.Gerec ve Yontemler: Akut toksisite calismasi OECD kilavuzlarina gore Z. gibbosa'nin etil asetat ve etanol (%70 v/v) ekstreleri icin yurutulmustur. Secilen bitki ekstrelerinin anti-diyabetik aktivitesi, sicanlar uzeinde olusturulan alloksan-nedenli diyabet modeli kullanilarak degerlendirilmistir.Bulgular: Zornia gibbosa Span ekstrelerinin uygulanan dozlarinda herhangi bir olum gorulmemistir. Ekstreler, alloksan-nedenli diabetes mellitus nedeniyle olusan fizyolojik degisiklikleri onemli olcude (pless than or equal to0.01) iyilestirmistir. 500 mg/kg konsantrasyonundaki sulu-alkollu ekstreler, standart ilac (glibenklamid) ile birlikte farkli konsantrasyonlardaki diger ekstrelerle karsilastirildiginda, daha yuksek aktivite gostermistir. Z gibbosa glukoz konsantrasyonunu onemli olcude dusurmus ve diyabetin degisik organlara hasar vermesi nedeniyle degisen enzim duzeylerini iyilestirmistir.Sonuc: Bu calismanin sonuclari, Z gibbosa'nin onemli anti-diyabetik etkiye sahip oldugunu gostermektedir. Bu nedenle, diyabet tedavisinde ve diyabetin neden oldugu diger saglik problemlerinde Z gibbosa'nin allopatik tip ile birlikte kullanilabilecegi ortaya konulmustur.Anahtar kelimeler: Zornia gibbosa Span, seker hastaligi, alloksan, glibenklamid