Ultra-high-performance liquid chromatography high-resolution mass spectrometry variants for metabolomics research

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Ultra-high-performance liquid chromatography high-resolution mass spectrometry variants for metabolomics research

Perez de Souza, Leonardo; Alseekh, Saleh; Scossa, Federico; Fernie, Alisdair R

Nature methods, 07/2021, Volume: 18, Issue: 7

Journal Article

Ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS) variants currently represent the best tools to tackle the challenges of complexity and lack of comprehensive coverage of the metabolome. UHPLC offers flexible and efficient separation coupled with high-sensitivity detection via HRMS, allowing for the detection and identification of a broad range of metabolites. Here we discuss current common strategies for UHPLC-HRMS-based metabolomics, with a focus on expanding metabolome coverage.

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Perez de Souza, Leonardo | Alseekh, Saleh | Scossa, Federico | Fernie, Alisdair R

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