There is a considerable current interest regarding specificity of the commonly used dyes that are used in a clinic biochemistry laboratory to estimate human serum albumin. It is in this context we have separated human serum albumin from left over plasma samples collected from human volunteers on Native PAGE followed by staining of albumin with various dyes with and without incubation of ester substrates prior to staining. We have observed that dyes like bromophenol blue is not specific for albumin and the observation with various Fast dyes are dye specific. Based on our result we feel that specific staining of albumin on gel is a candidate method for checking the specificity of the dyes for albumin.