Display omitted •PVA and FWEP/PVA blend films were prepared.•Physicochemical and antioxidant properties of different films were determined.•The FWEP/PVA films exhibited a potent antioxidant activities.•FWEP/PVA film enhanced the re-epithelization process.•FWEP/PVA film could be used as a promoter agent in healing process. Edible and biodegradable films from fenugreek (Trigonella foenum graecum) derived polysaccharide (FWEP) reinforced by poly (vinyl alcohol) (PVA) were prepared. Four films at different ratios of FWEP/PVA: F1 (70/30), F2 (50/50), F3 (30/70) and PVA (100% PVA) were characterized in terms of physical, structural, optical, thermal and morphological properties. The antioxidant activities of composite films were tested in vitro and evaluated in vivo through wound healing on CO2 laser fractional burn in rats. Morphological study of FWEP composite films showed a compact and homogenous structure. The thermal properties evaluated by differential scanning calorimetry showed a total miscibility between both polymers. X-ray diffraction and Fourier transform infrared spectroscopy indicated that FWEP/PVA films have semi-crystalline nature with a good compatibility. Results showed that F1 depicted the higher antioxidant activity in vitro, compared to other film ratios. In the same way, F1 exhibited potent percentage of wound healing activity revealed by less erythema, less crusting/scabbing, higher general wound appearance scores and a higher content of collagen (97.15 ± 57.72 mg/g of tissue), during eight days of treatment. Overall, obtained data demonstrated that FWEP/PVA composed films displayed strong wound healing potential presumably proved through its antioxidant activities.