Development of magnetic nanomaterials has greatly promoted the innovation of in-tube solid-phase microextraction. This review article gives an insight into recent advances in the modifications and applications of magnetic nanomaterials for in-tube solid-phase microextraction. Also, different magnetic nanomaterials which have recently been utilized as in-tube solid-phase microextraction sorbents are classified. This study shows that magnetic nanomaterials have gained significant attention owing to large specific surface area, selective absorption, and surface modification. Magnetic in-tube solid-phase microextraction has been applied for the analysis of food samples, biological, and environmental. However, for full development of magnetic in-tube SPME, effort is still needed to overcome limitations, such as mechanical stability, selectivity and low extraction efficiency. To achieve these objectives, research on magnetic in-tube SPME is mainly focused in the preparation of new extractive phases. Display omitted •Four groups of materials, used as coatings for magnetic in-tube SPME, were reviewed.•Silica and layered double hydroxides as coatings in magnetic in-tube SPME were investigated.•The roles of variable magnetic field in magnetic in-tube SPME are described.•Magnetic in-tube SPME techniques as well-known sample preparation methods were described.