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Yao, Hao-Ying-Ye; Wang, Jun-Qiao; Yin, Jun-Yi; Nie, Shao-Ping; Xie, Ming-Yong
Food research international, 20/May , Volume: 143Journal Article
Display omitted •Progress of NMR analysis in polysaccharide structure and conformation is summarized.•NMR spectra data of typical polysaccharides are collected.•NMR analysis in cell wall structure in situ and other applications are hotspots.•Challenges and perspertives of NMR analysis in polysaccharides are pointed out. Nuclear magnetic resonance (NMR) has been widely used as an analytical chemistry technique to investigate the molecular structure and conformation of polysaccharides. Combined with 1D spectra, chemical shifts and coupling constants in both homo- and heteronuclear 2D NMR spectra are able to infer the linkage and sequence of sugar residues. Besides, NMR has also been applied in conformation, quantitative analysis, cell wall in situ, degradation, polysaccharide mixture interaction analysis, as well as carbohydrates impurities profiling. This review summarizes the principle and development of NMR in polysaccharides analysis, and provides NMR spectra data collections of some common polysaccharides. It will help to promote the application of NMR in complex polysaccharides of biochemical interest, and provide valuable information on commercial polysaccharide products.
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