Microplastics (MPs), omnipresent contaminants in the ocean, could be carried by seawater intrusion into coastal aquifers, which might affect the fate of heavy metals existing in aquifers. Herein, we investigated the release behavior of arsenic (As) in coastal aquifers during MPs-containing seawater intrusion by applying laboratory experiment and numerical simulation. We found that seawater with marine MPs enhanced the release of As in aquifers, especially for dissolved As(V) and colloidal As. Negatively charged MPs competed with As(V) for the adsorption sites on iron (hydr)oxides in aquifers, resulting in the desorption of As(V). In addition, MPs could promote the release of Fe-rich colloids by imparting negative charge to its surface and providing it with sufficient repulsive force to detach from the matrix, thereby leading to the release of As associated with Fe-rich colloid. We also developed a modeling approach that well described the transport of As in coastal aquifer under the impact of MPs, which coupled variable density flow and kinetically controlled colloids transport with multicomponent reactive transport model. Our findings elucidated the enhancement of MPs on the release of As in aquifers during seawater intrusion, which provides new insights into the risk assessment of MPs in coastal zones. Display omitted •Marine MPs carried by seawater intrusion enhance As release in coastal aquifer.•MPs promote the release of dissolved As(V) due to the competitive adsorption.•MPs facilitate the mobilization of As bound to Fe-rich colloids.•A reactive model is constructed to describe As transport in coastal aquifer.