This study was aimed at exploring the carryover effect of short range of motion (RoM) isokinetic conditioning on vastus medialis (VM) motor unit recruitment (MUR) across the full RoM. Fifty-five women were randomly assigned to one of four groups: G1 ( n = 14) and G2 ( n = 14) trained concentrically at 30 and 90°/s, respectively whereas G3 ( n = 13) and G4 ( n = 14) trained similarly but using the eccentric mode. All 4 groups trained within 30–60° of knee flexion. The training protocol consisted of 4 sets of 10 maximal repetitions, 3 times a week for 6 weeks. sEMG was recorded from the VM for analysis of mean frequency of the EMG power spectrum prior to the training period and 2 days after its termination. The EMG assessments took place during dynamic contractions within 3 angular RoM’s: 85–60° (R1), 60–30° (R2) and 30–5° (R3). In addition MUR was evaluated during isometric contractions at 10°, 45° and 80°. Significant increases were observed in the MUR at R1, R2, and R3 during dynamic contractions as well as in all 3 angles during isometric contractions. These findings applied equally regardless of the mode of contraction and motion speed during training. The fact that MUR increased significantly within untrained RoM’s may point out to the potential benefits of short RoM conditioning, particularly in those cases where, during specific phases of rehabilitation, a wider RoM may be contraindicative.