This study aimed to isolate, identify, and evaluate the probiotic properties of species from honey of the stingless bee . spp. were isolated from five different meliponicultures, and the isolates were characterized through Gram-staining and a catalase test. Tolerance to acidic conditions and bile salt (0.3%), hydrophobicity, and autoaggregation tests were performed to assess the probiotic properties of the selected isolates, HTI-19 and HTI-23. Both isolates exhibited excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria and possessed significantly high survival rates in 0.3% bile solution for 3 h. Their survival rates in acidic conditions were also comparable to a commercial probiotic strain, GG. Interestingly, the hydrophobicity and autoaggregation percentage showed no significant difference from GG, a commercial probiotic strain. The results from this study suggest that HTI-19 and HTI-23 isolated from stingless bee honey have considerably good probiotic properties. Therefore, more studies should be done to investigate the effects of these bacteria cultures on gastrointestinal health.