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Spahn, Christian M.T.; Blaha, Gregor; Agrawal, Rajendra K.; Penczek, Pawel; Grassucci, Robert A.; Trieber, Catharine A.; Connell, Sean R.; Taylor, Diane E.; Nierhaus, Knud H.; Frank, Joachim
Molecular cell, 05/2001, Volume: 7, Issue: 5Journal Article
Tet(O) belongs to a class of ribosomal protection proteins that mediate tetracycline resistance. It is a G protein that shows significant sequence similarity to elongation factor EF-G. Here we present a cryo-electron microscopic reconstruction, at 16 Å resolution, of its complex with the E. coli 70S ribosome. Tet(O) was bound in the presence of a noncleavable GTP analog to programmed ribosomal complexes carrying fMet-tRNA in the P site. Tet(O) is directly visible as a mass close to the A-site region, similar in shape and binding position to EF-G. However, there are important differences. One of them is the different location of the tip of domain IV, which in the Tet(O) case, does not overlap with the ribosomal A site but is directly adjacent to the primary tetracycline binding site. Our findings give insights into the mechanism of tetracycline resistance.
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